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	Provedor de dados BJMBR (1) Autor Aird,S.D. (1) Boni-Mitake,M. (1) Nascimento,N. (1) Rogero,J.R. (1) Spencer,P.J. (1) Palavra-chave Bothrops jararacussu (1) Bothropstoxin-1 (1) Chromatography (1) Myotoxins (1) Purification (1) Tipo do documento Info:eu-repo/semantics/other (1) Ano 1998 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última A single-step purification of bothropstoxin-1 BJMBR Spencer,P.J.; Aird,S.D.; Boni-Mitake,M.; Nascimento,N.; Rogero,J.R.. Bothrops venoms are complex mixtures of components with a wide range of biological activities. Among these substances, myotoxins have been investigated by several groups. Bothropstoxin-1 (Bthtx-1) is a phospholipase A2-like basic myotoxin from Bothrops jararacussu. The purification of this component involves two chromatographic steps. Although providing a pure material, the association of these two steps is time consuming and a single-step method using high performance chromatography media would be useful. In the present study, we describe a single-step purification method for Bthtx-1. Bothrops jararacussu venom was dissolved in 1 ml buffer. After centrifugation, the supernatant was injected into a Resource-S cation exchange column connected to an FPLC... Tipo: Info:eu-repo/semantics/other Palavras-chave: Myotoxins; Purification; Chromatography; Bothrops jararacussu; Bothropstoxin-1. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900004 Registros recuperados: 1 Primeira ... 1 ... Última

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